The PRECISE Study:

Biomarkers and outcome predictors of pediatric nephrotic syndrome: a genetic, transcriptomic and secretome multiomics study

Financed under the European Joint Program on Rare Diseases (EJP-RD) call, the PRECISE study aims to identify novel non-invasive predictive biomarkers of the response to the treatment and the occurrence of relapses in children with idiopathic nephrotic syndrome (INS), using urine and blood as "liquid biopsies" in a large pediatric clinical cohort. The project will combine spectral flow cytometry, single-cell RNA sequencing, and a deep secretome multi-omics analysis.

The objectives are:

• The characterization of INS clinical spectrum- natural history of INS progression and treatments.
• The definition of the genetic/epigenetic modifications participating in the pathogenesis of INS.
• The evaluation of adaptive immune system alterations in INS children.
• The identification of specific protein patterns in serum and urine in different INS subtypes through multi-omics analyses.
• The generation of predictive models of INS manifestation and progression through the integration of -omics, genetic, and clinical data.