The PRECISE Study:
Biomarkers and outcome predictors of pediatric nephrotic syndrome: a genetic, transcriptomic and secretome multiomics study
Financed under the European Joint Program on Rare Diseases (EJP-RD) call, the PRECISE study aims to identify novel non-invasive predictive biomarkers of the response to the treatment and the occurrence of relapses in children with idiopathic nephrotic syndrome (INS), using urine and blood as "liquid biopsies" in a large pediatric clinical cohort. The project will combine spectral flow cytometry, single-cell RNA sequencing, and a deep secretome multi-omics analysis.
The objectives are:
- The characterization of INS clinical spectrum- natural history of INS progression and treatments.
- The definition of the genetic/epigenetic modifications participating in the pathogenesis of INS.
- The evaluation of adaptive immune system alterations in INS children.
- The identification of specific protein patterns in serum and urine in different INS subtypes through multi-omics analyses.
- The generation of predictive models of INS manifestation and progression through the integration of -omics, genetic, and clinical data.
Design: | Open, multicenter, non-pharmacological observational study. The PRECISE provides both retro- and prospective longitudinal records on clinical, genetic as well as laboratory data. |
Patients enrolled: | 110 treatment-naive onset INS children (discovery phase). |
Coordinating Center: | Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Pediatric Nephrology Dialysis and Transplantation Division, Milan, Italy |
Consortium Partners: | Italy: |
Additional Centers: | ERKNet European Rare Kidney Disease Reference Network ESCAPE European Paediatric Nephrology Centers |
Contact Information: | giovanni.montini@policlinico.mi.it federica.collino@unimi.it |
Active ESCAPE Participants: